Part:BBa_T2000:Design

7x His tag tail domain

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This is a 7x His tag rather than a standard 6x His tag because Tom Knight had heard anecdotally that 7x His is more effective than 6x His.

Used both His codons in order to avoid repeats. Also included a double TAATAA stop codon.

Source

This is a synthetic sequence.

References

<biblio>

1. Bornhorst pmid=11036646

</biblio>